Silkworm　fibers　have　attracted　widespread　attention　for　their　superb　glossy　texture　and　promising　mechanical　performance.　The　mechanical　properties　can　be　reinforced　with　carbon　nanofillers,　particularly　carbon　nanotubes　(CNTs),　depending　on　the　CNT　content　in　the　silk　fibers.　In　order　to　increase　the　CNT　content,　lignosulfonate　(LGS)　was　used　as　a　surfactant　to　ameliorate　the　CNT　solubility,　dispersibility,　and　biocompatibility.　The　resulting　CNT/LGS　nano-composite　was　further　processed　through　an　additional　purification　method　to　remove　excess　surfactant　and　enhance　the　CNT/LGS　ratio.　Then　the　purified　biocompatible　single　and　multiple-walled　CNTs　were　fed　to　silkworms,　leading　to　a　large　CNT　content　in　the　resulting　silk　fibers.　Reinforced　silk　fibers　were　produced　with　a　mechanical　strength　as　high　as　1.07　GPa　and　a　strain　of　16.8%.　The　toughness　modulus　is　1.69　times　than　that　of　the　unpurified　group.　The　CNT-embedded　silk　fibers　were　characterized　via　Raman　spectrometry　and　thermogravimetric　analysis　(TGA),　demonstrating　that　the　CNT　content　in　the　silk　fibers　increased　1.5-fold　in　comparison　to　the　unpurified　group.　The　increased　CNT　content　not　only　contributed　to　the　selfassembly　into　buffering　knots　of　silk　fibers,　but　it　also　enhanced　the　conductivity　of　graphitized　silk.　Our　coating　and　purification　strategies　provide　a　potential　facile　way　to　obtain　natural　silk　fibers　with　high　mechanical　performance.