In　order　to　establish　the　best　procedure　to　store　the　femur　samples　from　the　biomechanical　viewpoint,　we　compared　the　effects　of　different　storage　methods　on　the　mechanical　properties　of　mouse　femurs.　We　obtained　femurs　surgically　from　twenty　C57BL/6J　female　mice,　12　weeks　old,　and　randomly　divided　them　into　5　groups,　i.e.　fresh　control　group,　4%　paraformaldehyde　fixation　group,　4℃　storage　group,　-20℃　storage　group　and　-80℃　storage　group,　respectively,　with　five　mice　in　each　group.　For　the　three　low-temperature　storage　groups,　each　group　was　stored　for　1　week,　2　months,　6　months　at　their　respective　temperatures.　After　rewarming,　three-point　bending　test　was　performed　to　test　the　load　and　deflection　changes.　The　results　showed　that　both　the　elastic　modulus　and　deflection　decreased　significantly　in　the　4%　paraformaldehyde　group.　The　maximum　load　and　elastic　modulus　of　the　samples　in　the　4℃　group　after　one　week　storage　was　significantly　reduced;　The　mechanical　properties　were　close　to　the　fresh　control　group　in　the　-20℃　group　stored　for　2　months　but　the　maximum　load　was　also　reduced　after　6　months.　However,　mechanical　properties,　such　as　elastic　load,　maximum　load　and　elastic　modulus,　were　not　changed　obviously　in　the　-80℃　storage　group.　Accordingly,　-80℃　cryopreservation　had　little　influence　on　the　mechanical　properties　of　bone　tissues,　which　proved　that　the　temperature　-80℃　is　a　suitable　one　for　long-term　preservation.　©　2016,　Editorial　Office　of　Journal　of　Biomedical　Engineering.　All　right　reserved.