Translated Abstract
Pyrola are the entire plants of Pyrola calliantha H.Andres and Pyrola decorata H. Andres, which belong to the Pyrolaceae and the frequently used herbs took down by China pharmacopoeia. They have the functions of getting rid of rheumatism, strengthening bones and muscles, hemostasis and so on. They all belong to the genus of pyrola and are China peculiar medicinal plants. These plants of family have 29 genus and more than 70 species and distribute peculiarly in temperate zone and cold zone of Northern Hemisphere. China has 7 genus and 36 species, among which there are 27 genus of Pyrola, distributing peculiarly in north and south. The wild resources are very abundant.Pyrola are very widely used and the curative effective is definite. Study of toxicology shows: there have no adverse reactions while taking medicine of these plants for long term. The important literatures of Chinese medicine regard this medicine as delicacies during successive dynasties, the drug has the function of restoring qi tonifying the kidney, expelling wind and removing damness, promoting blood flow to regulate menstruation. The indications is as follows: weakness and cough, wrought and injury, hematemesis, rheumatism arthralgia, metrorrhagia and metrostaxis, traumata hemorrhageModern medical research shows: it can enhance body immunity, promote coronary circulation, enhance organic blood flow, and have the functions of broad spectrum antibiosis antiinflammation, which is the Chinese traditional medicine of notable curative effect in curing the sickness of cardiovascular, traumata hemorrhage. The mainly chemical compositions of Pyrola are as follows: pyrolin, meletin, galic acid, chimaphilin, homoarbutin, isohomoarbutin, N-phenyl-2-naphthylamine, renifolin, hyperin, 2″-O-galloyl hyperin, 6′-O-galloyl homoarbutin, amino acids, tannin, aetherolea and so on, The researches on chemical compositions of Pyrola provide foundation for the utilization and exploitation of Pyrola drug resource.Hyperlipidemia (HLP) is a kind of disorder, whose level of blood plasm cholesterol or triacylglycerol exceeds upper limit of normal range caused by the abnormality of fat metabolism or running. This disorder is the main risk factor of initiating atherosclerosis (AS) and coronary atherosclerotic heart disease (CHD).More and more researches show: Chinese tradition medicine has the predominance of the definite curative effect and fewer adverse reactions in preventing and curing HLP, so looking for lead compound from the naturally occurring drugs and developing the new drugs of highly active and harmfulless is the important direction for the researchers all over the world.Modern pharmacology research shows: Pyrola has the functions of cardio tonic, decompression, antiphlogistic, anticancer, anti-aging and improving symptom of coronary heart disease. Empirical study proves: Water extract of Pyrola could broaden ex vitro extremities, aures, brain blood vessel, and also could increase cardiac muscle trophicity blood stream, heighten plasma cAMP, clean free radical, depress cerebral vascular resistance etc.,This topic carry out researches on the function of lowering blood fat, which is on the basis of researches done by predecessor, adopting macroporous adsorptive resins of LSA-5B to separate the extraction of Pyrola, we get two parts of total flavonoids (30% - 50% ethanol elution part) and water solubility(20% ethanol elution part). Then adopting differ craft to proceed the farther purification of total flavones. We find found ethyl acetate extraction could greatly raise the content of total flavones, and the content of purification total flavones is 42.80% by the means of spectrophotometry. Adopting admixture standard to establish the assaying means of hyperin and 2＂-O-galloyl hyperin through the optimization of determining condition: [mobile phase: acetonitrile -0.05% potassium dihydrogen phosphate-0.05%phosphoric acid (20:30:85),wave length: 263nm, columntemperature:25kelve, flow rate:1mL• min-1], On the basis of pharmacology, we take mouse as the investigated subject, and adopt 75% eggnog to create hyperlipemia model, total flavones part and 20% ethanol elution part as test medicament. We find that the part of water solubility have the function of lowering blood fat, but the total flavones have no significant difference of lowering the mouse’s blood fat, These experiments establish foundation for further studies.
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