With　the　development　of　biological　analytical　techniques　and　high　throughput　screening　techniques,　a　large　number　of　automated　biological　agents　dispensing　systems　are　widely　used　in　the　field　of　life　science　research.　Non-contact　dispensing　method　characterizedby　its　small　dispensing　volume,　high　dispensing　precision　and　quick　service　speed　which　satisfies　the　requirements　of　biological　agent＇s　distribution,　becomes　the　mainstream　dispensing　method　used　in　reagent　dispensing　systems.　However,　the　difficult　control　way,　complex　system　of　non-contact　dispensing　method　and　its　vulnerable　dispensing　process,　which　can　be　easily　affected　by　characteristics　of　the　agent,　have　hindered　the　application　and　development　of　the　method.　In　this　study,　simulation　model　of　the　separation　process　ofmicro　scale　biological　reagent　are　constructed　in　order　to　solve　the　above　problems.　The　prerequisites　of　the　non-contact　dispensing　method,　the　relationship　between　dispensing　volume　and　precision　and　characteristics　of　reagents,　formation　of　system　and　control　parameters　are　researched　through　theoretical　analysis　and　numerical　simulations.　The　experiment　results　are　quite　consistent　with　the　simulation　results;　therefore　it　well　verifies　the　reliability　of　the　modeling　and　simulation　method　and　also　lays　a　firm　theoretical　foundation　for　the　system　design　of　non-contact　micro-liquid　reagent　distribution　and　control　optimization　©　Maxwell　Scientific　Organization,　2013.