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Author:

Li, Qi (Li, Qi.) | Wang, Maode (Wang, Maode.) | Wang, Ning (Wang, Ning.) | Wang, Jia (Wang, Jia.) | Qi, Lei (Qi, Lei.) | Mao, Ping (Mao, Ping.)

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SCIE PubMed Scopus

Abstract:

Accumulating evidence indicates microRNA-216b (miR-216b) plays an important role in the development and progression of various cancers. However, little is known about the function of miR-216b in gliomas. In this study, we aimed to investigate the expression level and functional significance of miR-216b in gliomas. We found that miR-216b was significantly downregulated in glioma specimens and cell lines. Overexpression of miR-216b suppressed the growth and migration of glioma cells, while miR-216b inhibition showed the opposite effects. Astrocyte elevated gene-1 (AEG-1) was predicted as a potential target gene of miR-216b by bioinformatics analysis. A dual-luciferase reporter assay showed that miR-216b could directly target the 3'-untranslated region of AEG-1. RT-qPCR and western blot analysis showed that miR-216 negatively regulated AEG-1 expression in glioma cells. Correlation analysis revealed an inverse correlation between miR-216b and AEG-1 in clinical glioma specimens. miR-216b also regulated the activation of nuclear factor-kappa B and Wnt signaling in glioma cells. Moreover, restoration of AEG-1 expression partially reversed the inhibitory effect of miR-216b overexpression on glioma cell growth and migration. Overall, these results revealed a tumor suppressive role of miR-216b in glioma tumorigenesis, and identified AEG-1 as a target gene of miR-216b action. Our study suggests that miR-216b can be potentially targeted for the development of novel therapies for gliomas.

Keyword:

NF-kappa B AEG-1 Wnt Glioma miR-216b

Author Community:

  • [ 1 ] [Li, Qi; Wang, Maode; Wang, Ning; Wang, Jia; Qi, Lei; Mao, Ping] Xi An Jiao Tong Univ, Dept Neurosurg, Affiliated Hosp 1, 277 Yanta West Rd, Xian 710061, Shaanxi, Peoples R China

Reprint Author's Address:

  • Xi An Jiao Tong Univ, Dept Neurosurg, Affiliated Hosp 1, 277 Yanta West Rd, Xian 710061, Shaanxi, Peoples R China.

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Source :

BIOMEDICINE & PHARMACOTHERAPY

ISSN: 0753-3322

Year: 2018

Volume: 104

Page: 420-426

3 . 7 4 3

JCR@2018

3 . 7 4 3

JCR@2018

ESI Discipline: PHARMACOLOGY & TOXICOLOGY;

ESI HC Threshold:14

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 2

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 6

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